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As a test for behavioral phenotyping of mutant mice, the light/dark transition test is one of the most widely used tests to measure anxiety-like behavior in mice (Crawley, 2000). The test is based on the natural aversion of mice to brightly illuminated areas and on their spontaneous exploratory behavior in novel environments (Crawley, 1985).
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The mouse light/dark box test. 2003 Feb 28;463 (1-3):55-65. doi: 10.1016/s0014-2999 (03)01274-3. The light/dark test is based on the innate aversion of rodents to brightly illuminated areas and on the spontaneous exploratory behaviour of rodents in response to mild stressors, that is, novel environment and light.
Light-dark box test. The light-dark box test (LDB) is a popular animal model used in pharmacology to assay unconditioned anxiety responses in rodents. [1] The extent to which behavior in the LDB measures anxiety is controversial.
The present results demonstrate that: 1) postpartum rats display fewer anxiety-related behaviors in a light-dark box than do diestrous virgins, 2) separating mothers from their pups 4 hours before testing in a light-dark box increases anxiety-related behaviors compared to dams not separated from their litter before testing, and 3) (+)-Bic and
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